The NCI-H441 cell line was established in 1982 from the pericardial fluid from a male patient with lung papillary adenocarcinoma. NCI-H441 cell line is hyperdiploid, has a modal chromosome number of 52, and can be cloned with or without serum in soft agar. NCI-H441 cells exhibit an epithelial morphology and adhere in culture. The NCI-H441 cell line expresses major surfactant apoprotein, mRNA, and pulmonary surfactant protein. NCI-H441 cells are suitable transfection hosts and can be used for lung cancer research and biomedical applications. An NCI-H441 transfection kit can be purchased through Altogen Biosystems to transfect NCI-H441 cells.
NCI-H441 cells were derived from the papillary adenocarcinoma of a human male patient and constitute an epithelial cell line that is one of the foremost models researchers have for investigating the efficacy of inhalant-based cancer therapies. With delivery to the lungs much easier through airways in the human body, novel treatments may focus on delivering nucleic acids in non-cytotoxic transfection reagents to alveoli and other lung cells. NCI-H441 cells are known for doubling in population size under sixty hours, and express the major surfactant apoprotein, making them a unique environment for drug testing, lung cancer research, and siRNA screening for the development of gene therapies targeting late-stage lung cancer.
Comments
Post a Comment