The NCI-H441 cell line was established in 1982 from the pericardial fluid from a male patient with lung papillary adenocarcinoma. NCI-H441 cell line is hyperdiploid, has a modal chromosome number of 52, and can be cloned with or without serum in soft agar. NCI-H441 cells exhibit an epithelial morphology and adhere in culture. The NCI-H441 cell line expresses major surfactant apoprotein, mRNA, and pulmonary surfactant protein. NCI-H441 cells are suitable transfection hosts and can be used for lung cancer research and biomedical applications. An NCI-H441 transfection kit can be purchased through Altogen Biosystems to transfect NCI-H441 cells.